The development of methods for the introduction of foreign genes into organisms has had a profound impact on the field of agriculture. While the movement of genes within plant species or between closely related plant species by traditional methods based on sexual reproduction has played an important role in crop improvement for most of this century, the pace of crop improvement by such methods has been slow and limiting due to the reliance on naturally occurring genes. The development of genetic transformation methods allows the introduction of recombinant DNA, into organisms. The recombinant DNA methods which have been developed have greatly extended the sources from which genetic information can be obtained for crop improvement. New crop plant varieties, developed through recombinant DNA methods, have reached the marketplace. Genetically engineered soybeans, maize, canola and cotton are now widely utilized by North America farmers.
Rapid progress has been made in developing the tools for manipulating genetic information in plants. Plant genes are being cloned, genetic regulatory signals deciphered, and genes transferred from entirely unrelated organisms to confer new agriculturally useful traits to crop plants. Recombinant DNA methods significantly increase the gene pool available for crop improvement. While the genetic sources for sequences for crop improvement have greatly expanded, the number of genotypes which can by stably transformed to recover a fertile plant are still limited. Therefore, methods which expand the scope of target recipient genotypes and/or improve recovery of fertile transformed plants from these genotypes are needed.